Cells which are expressing target gene

Cells which are expressing target gene

FIGURE 21.36 FISH To Detect and Measure mRNA Levels

A variety of different mRNA species are expressed at any one time within a tissue. Not all cells express the same genes or express them at the same level. If the target cells are probed with fluorescently labeled DNA (red), this will bind to the corresponding mRNA (blue). Notice that the probe DNA does not bind to the nuclear DNA because in this procedure the cells were not treated to denature the chromosomal DNA. The target gene in this example was only expressed in two of the cells.

Target DNA

Molecular beacon probe

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