On

FIGURE 25.08 Using Gene Fusions to Survey Regulation

The regulatory region of the target gene (green) controls the expression of the reporter structural gene (purple). Therefore, assaying the level of reporter enzyme reveals how the target gene would be expressed under the chosen conditions. In this example, the reporter gene is lacZ and the level of expression is monitored by the breakdown of ONPG to release yellow nitrophenol.

Regulatory regions may be analysed by deleting segments of the DNA and checking for effects on gene expression.

NO mRNA

No enzyme mRNA

b-galactosidase

b-galactosidase

Yellow color

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