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FIGURE 21.19 Use of 32P and 35S to Label Nucleic

Acids B. Phosphate versus phosphorothioate

FIGURE 21.19 Use of 32P and 35S to Label Nucleic

Acids B. Phosphate versus phosphorothioate

(A) Positioning of 32P in DNA. To make radioactive DNA, the phosphorus atom in the phosphate backbone is replaced with its radioactive isotope, 32P. (B) Phosphate versus phosphorothioate. Instead of replacing the phosphorus atom of the phosphate group, one of the oxygen atoms can be replaced by the radioactive isotope of sulfur, 35S, giving a phosphorothioate.

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