Making a Restriction

A diagram that shows the location of restriction enzyme cut sites on a segment of DNA is known as a restriction map. The first step in generating such a map is to restriction map A diagram showing the location of restriction enzyme cut sites on a segment of DNA T4 ligase Type of DNA ligase from bacteriophage T4 and which is capable of ligating blunt ends

FIGURE 22.05 DNA Ligase Joins Fragments of DNA

T4 DNA ligase connects the sugar-phosphate backbone of two pieces of DNA. In the example, overlapping sticky ends connect a double stranded piece of DNA, but the backbone of each strand has not been connected. T4 DNA ligase recognizes these nicks or breaks in the backbone and uses energy from the hydrolysis of ATP to drive the ligation reaction.

(bcoH

DNA Ligase (T4)

DNA Ligase

DNA Ligase

Was this article helpful?

0 0

Post a comment