Location In Membrane

Target Regulatory Coding region gene region

Reporter Regulatory Coding region gene region

FIGURE 25.07 Construction of a Gene Fusion

Creating gene fusions help in investigating how genes are regulated. The regulatory region of the target gene is joined to the reporter gene coding region. The reporter enzyme will now be made under conditions where the target gene would normally be expressed.

Cut and rejoin

Hybrid gene or

"gene fusion"

Target regulatory-region -

-Reporter coding region

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