Target sequence

3' Newly made DNA

3' Newly made DNA

5' Target sequence

Newly made DNA 3'

Target sequence 5'

FIGURE 23.07 The Third Cycle of the PCR

The products from the second cycle go through the same process as before. The four double-stranded pieces are denatured into eight single-stranded pieces. The primers anneal and DNA polymerase makes the complementary strands. After this cycle, the number of sequences containing only the target DNA grows exponentially, far exceeding any other product shown in this figure.


Newly made DNA

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