Info

5' TAAGGTCTCG

Newly made DNA

3'

3'

_aTTCCAGAGC.-

Target sequence

5'

FIGURE 23.06 The Second Cycle of the PCR

The entire cycle is repeated starting with the two DNA pieces produced in the first cycle. The two double-stranded pieces of DNA are denatured into four single-stranded pieces at 90°C. The temperature drops to 50°C in order for the primers to anneal. Finally, the polymerase extends the DNA at 70°C to convert the four single-stranded templates into double-stranded DNA.

Starting materials

5 Target sequence

3' Target sequence

Target sequence 3

Target sequence 5'

1. Separate strands - 90°g

Products

5 Target sequence

5 Target sequence

Newly made DNA

5'

Newly made DNA

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