5 6 7 8 M 9 10

FIGURE 23.13 Identification of Fungal Pathogens by RAPD

RAPD banding patterns generated using the 10 base primer, AACGCGCAAC, on genomic DNA of five closely related strains of the pathogen Botrytis cinerea (lanes 1-5), three other strains from the genus Botrytis (lanes 6, 7 & 8) and several less related harmless fungi Alternaria (9), Aspergillus (10), Cladosporium (11), Epicoccum (12), Fusarium (13), Hainesia (14), Penicillium (15), Rhizoctonia (16 & 17), and the host plant, strawberry (18). Lane 0: negative control (no DNA). Lane M: molecular mass marker. From: Rigotti et al., FEMS Microbiology Letters (2002) 209:169-174.

Reverse transcription followed by PCR allows cloning of genes starting from the messenger RNA.

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