Fluorescence in Situ Hybridization FISH

All the previous techniques require the scientist to isolate the DNA, RNA or protein from its cellular environment. In contrast, Fluorescence in Situ Hybridization, better known as FISH, is used to detect the presence of a gene, or the corresponding messenger RNA, within the actual cell (Fig. 21.34). DNA sequences from the gene of interest must first be generated for use as a probe. These may be obtained by cloning the gene or, more usually nowadays, amplified by PCR (see Ch. 23 for details). In

FISH See Fluorescence in Situ Hybridization

Fluorescence in Situ Hybridization (FISH) Using a fluorescent probe to visualize a molecule of DNA or RNA in its natural location South-Western blotting Detection technique in which a DNA probe binds to a protein target molecule

Zoo blotting Comparative Southern blotting using DNA target molecules from several different animals to test whether the probe DNA is from a coding region

DNA or RNA sequences may be detected in their natural location inside the cell by hybridization to fluorescent probes.

DNA probe

DNA probe

Denature, mix

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