Dna

mRNA III 1

FIGURE 21.28 R-Loop Analysis to Visualize Introns

R-loop analysis can be used to visualize the introns of eukaryotic genes. First, a double-stranded DNA molecule is denatured into two single strands. One strand is annealed to the corresponding mRNA. Because RNA lacks introns the DNA and RNA anneal only in the coding regions. The introns, which are only found in the DNA, remain single-stranded and loop out from the heteroduplex. The entire complex can be visualized by electron microscopy after shadowing with metal ions.

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