FIGURE 23.18 Synthesis of Hybrid Gene by Using Overlap Primers

Overlapping primers can be used to link two different gene segments. In this scheme, the overlapping primer has one end with sequences complementary to target sequence 1, and the other half similar to target sequence 2. The PCR reaction will create a product with these two regions linked together.

Eventual product

Target sequence 1 Target sequence 2

Mutations may be inserted artificially into DNA by altering a few bases in a PCR primer.

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