DNA Replication Is a Two Stage Process Occurring at the Replication Fork

Replication is the process by which the DNA of the ancestral cell is duplicated, prior to cell division. Upon cell division, each of the descendants will get one complete copy of the DNA that is identical to its predecessor. The first stage in replication is to separate the two DNA strands of the parental DNA molecule. The second stage is to build two new strands, using each of the two original strands as templates. The most fundamental aspect of replication is the base pairing of A with T and of G with C. Each of asexual or vegetative reproduction Form of reproduction in which there is no reshuffling of the genes between two individuals replication Duplication of DNA prior to cell division template strand Strand of DNA used as a guide for synthesizing a new strand by complementary base pairing

Supercoiling Causes Problems for Replication 105

FIGURE 5.01 Template Strand and Base Pairing in DNA Replication

Incoming nucleotides line up on the template strand and are then linked together to form the new strand of DNA. The arriving nucleotides are positioned by base pairing, in which A pairs with T and G binds to C. These base pairs are held together with hydrogen bonds.

New DNA is made using the existing strands as templates for base pairing.

Each daughter cell gets one old strand and one new strand of DNA.

Template strand

Nucleotide precursor about to bind

Incoming bases forming new strand

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