Scanning Transmission Electron Microscopy for Biology

Summary 13-1

13.1 Introduction 13-2

13.2 Overview of High-Resolution 3D Imaging

Techniques for Biology 13-3

Confocal Laser Microscopy • X-Ray, NMR, and Other • Electron Tomography

13.3 From the First STEM to Aberration Correction 13-6

The First STEM • The STEM Imaging with Several Parallel Detector Signals • Reciprocity • Phase Contrast versus Scatter Contrast • Aberration-Corrected STEM • 3D STEM

13.4 Resolution of 3D STEM on Biological Samples 13-12

Radiation Dose • Blur • Scatter Contrast • Detection of an Embedded Staining Particle • Confidence Level of Detection • Dose-Limited Resolution • Dose-Limited Resolution in Focal Series

13.5 Initial Experimental Results on a Biological Sample 13-18

Focal Series of a Conventional Thin Section • Deconvolution •

Deconvolved Images

13.6 Future Outlook 13-20

13.7 Comparison of 3D STEM with TEM

Tomography for Biology 13-21

13.8 Conclusions 13-21

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