Nanopipettes

Micromachined nanopipettes provide an alternative strategy to fabricate biomolecular nanopatterns (Figure 15.3B). Nanopipettes were used to deliver protein G and green fluorescent protein (GFP) onto aldehyde- and BSA-coated glass slides, respectively [183]. In both cases, the attachment between the protein and the substrate was strong enough for AFM imaging. This method can potentially produce protein patterns 1000 times smaller than those constructed with conventional microarray printers. In addition, the nanopipette system can be directly incorporated with high-performance liquid chroma-tography (HPLC), which should enable multiple protein species to be patterned on the surface [183].

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