Semicomparative and Continuous Data Acquisition Techniques

The low dynamic range of 2D gel electrophoresis, and its limitations with respect to druggable target discovery, have led some researchers to exclude the comparative step altogether. Instead, traditional one-dimensional (1D) Laemlli gels have been used to separate specifically enriched pools of proteins, which would not be resolved by 2D electrophoresis. The 1D gel is principally a deconvolution step to facilitate mass spec throughput, although additional information on the proteins can be inferred from molecular weight and the distribution and frequency of annotation, which provides semi-quantitative data comparable with expression patterns derived from cDNA libraries (e.g., TIGR expression profiles 29).

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