The simulations that were presented in Chapter 7 allowed a comparison of the behavior of the model of erythrocyte metabolism with real experimental results (see the original papers(1-4) for the data). While this was an extremely important step in model verification, it left many unanswered questions about the underlying regulatory and control features. In Chapter 5 we introduced the concepts of MCA and described how they provide greater insights into a model's behavior than would be unattainable with only descriptive language. In this chapter we apply the procedures of MCA to our model.
The main questions that are addressed are: Which reactions are important for controlling pathway fluxes? Which reactions are important for controlling metabolite concentrations? What is the mechanism for the control of 2,3-BPG concentration?
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