Eisenthal and Cornish Bowden equation direct linear plot

A totally different approach to analyzing enzyme kinetic data involves plotting many lines onto the experimental data; it was introduced in 1974 by Eisenthal and Cornish-Bowden.(3) For this procedure a Cartesian a-is-system is drawn with v0 on the ordinate and [A]0 on the abscissa. With the data pairs ([A]0i,v0i), i = 1,...,N, a straight line is drawn to pass through the points (-[A]0i, 0) and (0, v0i). The intersection of this line with others, similarly drawn, occurs at as many different points as there are data pairs. In other words, since experimental data are never error free, a set of values {Km, Vma-}j, j = 1,...,N-1, is obtained. The ne-t step in the analysis is to arrange the respective values of Km,j and Vma-,j into two separate sets, in ascending order. It has been shown that the 'statistically best estimate' of the two parameters is given by the respective median (middle) values in the set.®

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Responses

  • dominik
    How to plot eisenthal cornish bowden?
    2 years ago
  • tauno
    What does a eisenthal cornishbowden plot look like?
    10 months ago
  • Mary
    How to determine values for eisenthalcornishbowden plot?
    10 months ago
  • Bilcuzal
    How to plot eisenthal and crownish plot?
    5 months ago

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