Elements of Enzyme Kinetics

2.1 Kinetics of Enzymic Reactions

2.1.1 Purpose

2.1.2 The Michaelis-Menten equation

2.1.3 Graphical evaluation of Fmax and Km

2.1.4 Lineweaver-Burk plot

2.1.5 Eadie-Hofstee plot

2.1.6 Hanes-Woolf plot

2.1.7 Eisenthal and Cornish-Bowden equation (direct linear plot)

2.2 Enzyme Inhibition

2.2.1 Degree of inhibition

2.2.2 Michaelis-Menten equations that include inhibitor effects

2.3 Enzyme Mechanisms

2.3.1 Michaelis-Menten mechanism

2.3.2 The steady state

2.3.3 Reversible Michaelis-Menten enzyme

2.4 Regulatory Enzymes

2.5 Exercises

2.6 References

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