Uas

Transfect yeast cells with genes encoding ^ r bait and fish hybrids

Co-activators and transcription pre-initiation complex

HIS mRNA

Transfect yeast cells with genes encoding ^ r bait and fish hybrids

Co-activators and transcription pre-initiation complex

HIS mRNA

(c) Fishing for proteins that interact with bait domain

Bait gene

Bait vector

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