Q

Folded protein

▲ FIGURE 16-6 Synthesis of secretory proteins and their cotranslational translocation across the ER membrane. Steps 1 , 2: Once the ER signal sequence emerges from the ribosome, it is bound by a signal-recognition particle (SRP). Step 3 : The SRP delivers the ribosome/nascent polypeptide complex to the SRP receptor in the ER membrane. This interaction is strengthened by binding of GTP to both the SRP and its receptor. Step 4 : Transfer of the ribosome/nascent polypeptide to the translocon leads to opening of this translocation channel and insertion of the signal sequence and adjacent segment of the growing polypeptide into the central pore. Both the SRP and SRP receptor, once dissociated from the translocon, hydrolyze their bound GTP and then are ready to

Folded protein initiate the insertion of another polypeptide chain. Step 5 : As the polypeptide chain elongates, it passes through the translocon channel into the ER lumen, where the signal sequence is cleaved by signal peptidase and is rapidly degraded. Step 6 : The peptide chain continues to elongate as the mRNA is translated toward the 3' end. Because the ribosome is attached to the translocon, the growing chain is extruded through the translocon into the ER lumen. Steps 7 , 8 : Once translation is complete, the ribosome is released, the remainder of the protein is drawn into the ER lumen, the translocon closes, and the protein assumes its native folded conformation.

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