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Alprenolol (AP)

labeled alprenolol is determined. In a plot of the inhibition of [3H]alprenolol binding versus epinephrine or isoproterenol concentration, such as shown here, the concentration of the competitor that inhibits alprenolol binding by 50 percent approximates the Kd value for competitor binding. Note that the concentrations of competitors are plotted on a logarithmic scale. The Kd for binding of epinephrine to its receptor on liver cells is only =5 X 10~5 M and would not be measurable by a direct binding assay with [3H]epinephrine. The Kd for binding of isoproterenol, which induces the normal cellular response, is more than tenfold lower.

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