Most Lipids and Many Proteins Are Laterally Mobile in Biomembranes

In the two-dimensional plane of a bilayer, thermal motion permits lipid molecules to rotate freely around their long axes and to diffuse laterally within each leaflet. Because such movements are lateral or rotational, the fatty acyl chains remain in the hydrophobic interior of the bilayer. In both natural and ar-

proportional to the fraction of labeled molecules that are mobile in the membrane. (b) Results of FRAP experiment with human hepatoma cells treated with a fluorescent antibody specific for the asialoglycoprotein receptor protein. The finding that 50 percent of the fluorescence returned to the bleached area indicates that 50 percent of the receptor molecules in the illuminated membrane patch were mobile and 50 percent were immobile. Because the rate of fluorescence recovery is proportional to the rate at which labeled molecules move into the bleached region, the diffusion coefficient of a protein or lipid in the membrane can be calculated from such data. [See Y I. Henis et al., 1990, J. Cell Biol. 111:1409.]

Membrane protein Ruoresœn1: reprit: Bleached area

Membrane protein Ruoresœn1: reprit: Bleached area

(b)
Time (s)

tificial membranes, a typical lipid molecule exchanges places with its neighbors in a leaflet about 107 times per second and diffuses several micrometers per second at 37 °C. These diffusion rates indicate that the viscosity of the bilayer is 100 times as great as that of water—about the same as the viscosity of olive oil. Even though lipids diffuse more slowly in the bilayer than in an aqueous solvent, a membrane lipid could diffuse the length of a typical bacterial cell (1 ^m) in only 1 second and the length of an animal cell in about 20 seconds.

The lateral movements of specific plasma-membrane proteins and lipids can be quantified by a technique called fluorescence recovery after photobleaching (FRAP). With this method, described in Figure 5-6, the rate at which membrane lipid or protein molecules move—the diffusion coefficient— can be determined, as well as the proportion of the molecules that are laterally mobile.

The results of FRAP studies with fluorescence-labeled phospholipids have shown that, in fibroblast plasma membranes, all the phospholipids are freely mobile over distances of about 0.5 ^m, but most cannot diffuse over much longer distances. These findings suggest that protein-rich regions of the plasma membrane, about 1 ^m in diameter, separate lipid-rich regions containing the bulk of the membrane phospholipid. Phospholipids are free to diffuse within such a region but not from one lipid-rich region to an adjacent one. Furthermore, the rate of lateral diffusion of lipids in the plasma membrane is nearly an order of magnitude slower than in pure phospholipid bilayers: diffusion constants of 10~8 cm2/s and 10~7 cm2/s are characteristic of the plasma membrane and a lipid bilayer, respectively. This difference suggests that lipids may be tightly but not irreversibly bound to certain integral proteins in some membranes.

10 Ways To Fight Off Cancer

10 Ways To Fight Off Cancer

Learning About 10 Ways Fight Off Cancer Can Have Amazing Benefits For Your Life The Best Tips On How To Keep This Killer At Bay Discovering that you or a loved one has cancer can be utterly terrifying. All the same, once you comprehend the causes of cancer and learn how to reverse those causes, you or your loved one may have more than a fighting chance of beating out cancer.

Get My Free Ebook


Post a comment