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M FIGURE 10-13 Model for reverse transcription of retroviral genomic RNA into DNA. In this model, a complicated series of nine events generates a double-stranded DNA copy of the single-stranded RNA genome of a retrovirus (top). The genomic RNA is packaged in the virion with a retrovirus-specific cellular tRNA hybridized to a complementary sequence near its 5' end called the primer-binding site (PBS). The retroviral RNA has a short direct-repeat terminal sequence (R) at each end. The overall reaction is carried out by reverse transcriptase, which catalyzes polymerization of deoxyribonucleotides. RNaseH digests the RNA strand in a DNA-RNA hybrid. The entire process yields a double-stranded DNA molecule that is longer than the template RNA and has a long terminal repeat (LTR) at each end. The different regions are not shown to scale. The PBS and R regions are actually much shorter than the U5 and U3 regions, and the central coding region is very much longer than the other regions. [See E. Gilboa et al., 1979, Cell 18:93.]

Gal-responsive Ty

Transform yeast cells; grow in galactose- and nongalactose-containing media

Gal-responsive Ty with unrelated added intron

Gal-responsive Ty

Transform yeast cells; grow in galactose- and nongalactose-containing media

Gal-responsive Ty with unrelated added intron

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