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a. Which tissues contain a binding activity that recognizes the sequence upstream of gene X? Is the transcription factor the same in each tissue?

b. If the binding activity was purified, what test could be done to verify that this factor is in fact a transcription factor?

c. What type of assay would be performed to determine the specific DNA sequence(s) to which the transcription factor binds?

d. If gene X is transcribed in lung and brain tissue but not in bone and skin tissue, what type of transcription factor is the binding activity? Speculate as to the identity of other factors that might be complexed at the gene X promoter in bone and skin tissue.

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