Info

Layer sample on column

(b) Ion-exchange chromatography

Layer sample on column

Negatively charged protein

Positively charged protein

Negatively charged protein

Positively charged protein

Positively charged gel bead

Elute negatively charged protein with salt solution (NaCl) 3

Positively charged gel bead

Elute negatively charged protein with salt solution (NaCl) 3

Layer sample on column

Collect fractions

Wash

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