Protein-coding region

Inverted repeat («50-bp)

Protein-coding region

Target-site direct repeat (5-11-bp)

▲ FIGURE 10-9 General structure of bacterial IS elements.

The relatively large central region of an IS element, which encodes one or two enzymes required for transposition, is flanked by an inverted repeat at each end. The sequences of the inverted repeats are nearly identical, but they are oriented in opposite directions. The sequence is characteristic of a particular IS element. The 5' and 3' short direct (as opposed to inverted) repeats are not transposed with the insertion element; rather, they are insertion-site sequences that become duplicated, with one copy at each end, during insertion of a mobile element. The length of the direct repeats is constant for a given IS element, but their sequence depends on the site of insertion and therefore varies with each transposition of the IS element. Arrows indicate sequence orientation. The regions in this diagram are not to scale; the coding region makes up most of the length of an IS element.

Donor DNA

Target DNA

9-bp target site

Transposase makes blunt-ended cuts in donor DNA and staggered cuts in target DNA

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