Info

Microtubule alignment

(-) end-directed movement

Centrosome separation

Pushing forces in overlap zone

Centrosome separation

Pushing forces in overlap zone

Pulling force on asters

▲ EXPERIMENTAL FIGURE 20-35 Cytosolic dynein participates in the formation and stabilization of mitotic spindle poles. In vitro reconstituted spindles were stained with fluorescently-labeled polyclonal antibodies to tubulin (green) and dynein (red) and examined with a fluorescence microsope. In the control spindle (left), cytosolic dynein is present at each tapered end, as well as at the equator. Addition of a dynein monoclonal antibody after the formation of the spindle (right) disrupts dynein localization and causes the poles to splay. [From R. Heald et al., 1997, J. Cell Biol. 138:615; courtesy of R. Heald.]

▲ FIGURE 20-36 Capture of chromosomes by microtubules in prometaphase. (a) In late prophase, spindle microtubules probe randomly for chromosomes by alternately growing and shrinking at their distal (+) ends. (b) Some chromosomes first encounter the side ( 1 ), not the end, of a microtubule, interacting with the microtubule through proteins at the kinetochore. Kinetochore-associated (+) end-directed motor proteins (e.g., MCAK) then move the chromosome to the (+) end ( 2|), thereby stabilizing the microtubule.

(a) Motor-generated forces CENP-E

(b) Treadmilling f

10 Ways To Fight Off Cancer

10 Ways To Fight Off Cancer

Learning About 10 Ways Fight Off Cancer Can Have Amazing Benefits For Your Life The Best Tips On How To Keep This Killer At Bay Discovering that you or a loved one has cancer can be utterly terrifying. All the same, once you comprehend the causes of cancer and learn how to reverse those causes, you or your loved one may have more than a fighting chance of beating out cancer.

Get My Free Ebook


Post a comment