Gm1

PLAP Raft

Cholera toxin AntibodiesY

Cholera toxin AntibodiesY

Copatch

Cholera toxin ♦

AntibodiesY

Separate patches

Cholera toxin ♦

AntibodiesY

Separate patches

▲ EXPERIMENTAL FIGURE 5-10 Some membrane lipids and proteins colocalize in lipid rafts. The results of biochemical studies suggested that GM1, a glycosphingolipid, and placental alkaline phosphatase (PLAP), a lipid-anchored membrane protein, aggregate together into lipid rafts, whereas the transferrin receptor (TfR), which traverses the entire membrane, does not. To locate these components in the intact plasma membrane, cells were treated with fluorescence-labeled cholera toxin (green), which cross-links closely spaced GM1 molecules, and with fluorescence-labeled antibodies (red) specific for either PLAP or TfR. Each antibody can cross-link closely spaced

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