Another approach to viewing cells comes from studying how they change during development of a complex organism. Bacteria, algae, and unicellular eukaryotes (protozoans, yeasts) often, but by no means always, can work solo. The concerted actions of the trillions of cells that compose our bodies require an enormous amount of communication and division of labor. During the development of multicellular organisms, differentiation processes form hundreds of cell types, each specialized for a particular task: transmission of electrical signals by neurons, transport of oxygen by red blood cells, destruction of infecting bacteria by macrophages, contraction by muscle cells, chemical processing by liver cells.
Many of the differences among differentiated cells are due to production of specific sets of proteins needed to carry out the unique functions of each cell type. That is, only a subset of an organism's genes is transcribed at any given time or in any given cell. Such differential gene expression at different times or in different cell types occurs in bacteria, fungi, plants, animals, and even viruses. Differential gene expression is readily apparent in an early fly embryo in which all the cells look alike until they are stained to detect the proteins encoded by particular genes (Figure 1-24). Transcription can change within one cell type in response to an external signal or in accordance with a biological clock; some genes, for instance, undergo a daily cycle between low and high transcription rates.
▲ FIGURE 1-24 Differential gene expression can be detected in early fly embryos before cells are morphologically different. An early Drosophila embryo has about 6000 cells covering its surface, most of which are indistinguishable by simple light microscopy. If the embryo is made permeable to antibodies with a detergent that partially dissolves membranes, the antibodies can find and bind to the proteins they recognize. In this embryo we see antibodies tagged with a fluorescent label bound to proteins that are in the nuclei; each small sphere corresponds to one nucleus. Three different antibodies were used, each specific for a different protein and each giving a distinct color (yellow, green, or blue) in a fluorescence microscope. The red color is added to highlight overlaps between the yellow and blue stains. The locations of the different proteins show that the cells are in fact different at this early stage, with particular genes turned on in specific stripes of cells. These genes control the subdivision of the body into repeating segments, like the black and yellow stripes of a hornet. [Courtesy of Sean Carroll, University of Wisconsin.]
Producing different kinds of cells is not enough to make an organism, any more than collecting all the parts of a truck in one pile gives you a truck. The various cell types must be organized and assembled into all the tissues and organs. Even more remarkable, these body parts must work almost immediately after their formation and continue working during the growth process. For instance, the human heart begins to beat when it is less than 3 mm long, when we are mere 23-day-old embryos, and continues beating as it grows into a fist-size muscle. From a few hundred cells to billions, and still ticking.
In the developing organism, cells grow and divide at some times and not others, they assemble and communicate, they prevent or repair errors in the developmental process, and they coordinate each tissue with others. In the adult organism, cell division largely stops in most organs. If part of an organ such as the liver is damaged or removed, cell division resumes until the organ is regenerated. The legend goes that Zeus punished Prometheus for giving humans fire by chaining him to a rock and having an eagle eat his liver. The punishment was eternal because, as the Greeks evidently knew, the liver regenerates.
Developmental studies involve watching where, when, and how different kinds of cells form, discovering which signals trigger and coordinate developmental events, and understanding the differential gene action that underlies differentiation (Chapters 15 and 22). During development we can see cells change in their normal context of other cells. Cell biology, biochemistry, cell biology, genetics, and ge-nomics approaches are all employed in studying cells during development.
Was this article helpful?
Learning About 10 Ways Fight Off Cancer Can Have Amazing Benefits For Your Life The Best Tips On How To Keep This Killer At Bay Discovering that you or a loved one has cancer can be utterly terrifying. All the same, once you comprehend the causes of cancer and learn how to reverse those causes, you or your loved one may have more than a fighting chance of beating out cancer.