Reconstituted vesicles capable of both electron transport and ATP synthesis
▲ EXPERIMENTAL FIGURE 8-25 Mitochondrial F-, particles are required for ATP synthesis, but not for electron transport. "Inside-out" membrane vesicles that lack F1 and retain the electron transport complexes are prepared as indicated. Although these can transfer electrons from NADH to O2, they cannot synthesize ATP! The subsequent addition of F1 particles reconstitutes the native membrane structure, restoring the capacity for ATP synthesis. When detached from the membrane, F1 particles exhibit ATPase activity.
face. As demonstrated in the experiment depicted in Figure 8-25, submitochondrial vesicles from which F1 is removed by mechanical agitation cannot catalyze ATP synthesis; when Fj particles reassociate with these vesicles, they once again become fully active in ATP synthesis. Because F1 separated from membranes is capable of catalyzing ATP hydrolysis, it has been called the F1 ATPase; however, its function in the cells is to synthesize ATP. We examine how it does so in the next section.
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