Zinc Metalloneurochemistry Physiology Pathology and Probes

Christopher J. Chang and Stephen J. Lippard*

Department of Chemistry, Massachusetts Institute of Technology, 77 Masachusetts Avenue, Cambridge, MA 02139, USA <[email protected]>

1 INTRODUCTION 322

2 ZINC IN THE BRAIN 323

2.1 Tissue Concentrations, Distribution, and Speciation 323

2.2 Transport, Trafficking, and Homeostasis 324

2.3 Labile Zinc as a Signal or Toxin 326

3 ZINC SENSING FOR NEUROSCIENCE APPLICATIONS 328

3.1 Overview of Zinc Detection Methods 328

3.2 Requirements for Effective Fluorescence Imaging of

Neuronal Zinc 329

4 BIOMOLECULE FLUORESCENT PROBES FOR ZINC 329

4.1 Peptide Sensors 329

4.2 Protein Sensors 335

4.3 Nucleic Acid Sensors 338

5 SMALL-MOLECULE FLUORESCENT PROBES FOR ZINC 339

5.1 Intensity-based Fluorescent Sensors with Ultraviolet Excitation 339

5.1.1 Quinoline Sensors 339

5.1.2 Dansyl Ionophores 341

5.1.3 Coumarin Fluorophores 341

5.1.4 Lanthanide-containing Probes 343

5.2 Intensity-based Fluorescent Sensors with Visible

Excitation, Including Xanthenones 344

5.2.1 Zinpyr Sensors 344

5.2.2 ZnAF Dyes 348

Metal Ions in Life Sciences, Volume 1 Edited by Astrid Sigel, Helmut Sigel and Roland K. O. Sigel © 2006 John Wiley & Sons, Ltd

5.2.3 Visible Excitation Calcium Probes Adapted for Intensity-based Zinc Sensing 349

5.2.4 Moderate-affinity Zinc Fluorophores 350

5.2.5 Materials-based Probes 353 5.3 Fluorescent Sensors for Ratiometric Detection of Zinc 354

5.3.1 Ultraviolet Calcium and Magnesium Probes Adapted for Ratiometric Zinc Sensing 354

5.3.2 Benzazole Probes 355

5.3.3 Ratiometric Zinc Sensing with Visible Excitation 356 6 CONCLUDING REMARKS AND FUTURE DIRECTIONS 359

ACKNOWLEDGMENTS 360

ABBREVIATIONS 360

REFERENCES 361

Was this article helpful?

0 0
Detoxify the Body

Detoxify the Body

Need to Detoxify? Discover The Secrets to Detox Your Body The Quick & Easy Way at Home! Too much partying got you feeling bad about yourself? Or perhaps you want to lose weight and have tried everything under the sun?

Get My Free Ebook


Post a comment