1 Place in 70% ethanol: 30min.
2 Transfer to two changes of 70% ethanol: 5min each.
3 Wash under a gently running tap: 10min.
4 Place in working solution of haematoxylin: 5min.
5 Wash in running tap water: 10min.
6 Dip slide in acid-alcohol for a few seconds.
7 Wash in running tap water: 10min.
8 Transfer to two changes of 70% ethanol: 5min each.
9 Transfer to 95% ethanol: 5min.
10 Place in absolute ethanol: 5min.
11 Transfer the slide to Histoclear: 3min.
Examine under the light microscope using 10*, 40*, and 100* objectives for at least 100 fields. Measure objects with the calibrated eyepiece micrometer. Nuclear chromatin and chromidial bars are black, other structures, varying shades of gray to black.
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