Reagents

• Iron haematoxylin.

• Solution 1: haematoxylin 10g dissolved in 1000ml 99.5% ethanol. Allow the solution to stand in light for 1 week. Store in brown glass-stoppered bottle.

• Solution 2: Ferrous ammonium sulphate 10g, Ferric ammonium sulphate 10g, concentrated hydrochloric acid 10ml, distilled water 100ml. Shelf life, 6 months.

For use: mix equal parts of each solution. The mixture keeps about 1 week.

• Acid-alcohol: 1% hydrochloric acid in 70% ethanol.

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