Procedure

Gloves are worn and forceps used to handle the specimen.

1 Place small pieces of tissue into 20ml of acid pepsin reagent. Incubate overnight at 37°C.

2 Transfer the contents of the bottle to two centrifuge tubes. Centrifuge at 500g for 2-5min.

3 Discard the supernatant.

4 Add a small amount of 10% formalin, mix, resuspend the pellet and fill the tubes with fixative.

5 Centrifuge the tubes again and discard the supernatant.

6 Transfer the deposits to glass slides. Cover with coverslips and examine each preparation under the microscope using the 10* objective and reduced light for Trichinella larvae.

0 0

Post a comment