Procedure

1 Record the macroscopical appearance. With unfixed specimens add an equal amount of formalin solution. Allow to react for 30min.

2 Mix the specimen. Transfer 10ml to a centrifuge tube. Centrifuge 10min at 800g.

3 Discard the supernatant.

4 Examine the whole deposit under the microscope using the 10* objective and the 40* objective where necessary.

Record the presence of protoscolices and hooklets. If necessary, compare with a reference specimen. A

negative result does not exclude hydatid disease.

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