Preparation of specimen

1 Filter some of the fixed specimen through a layer of dampened surgical gauze into a beaker.

2 Place about 3ml of the filtered specimen into a centrifuge tube and fill the tube with 0.9% saline.

3 Centrifuge for 1min at 800g.

4 Pour off enough of the supernatant so that about 1ml remains in the tube. Dilute with 0.9% saline if the specimen appears too thick.

5 Mix a drop of egg albumin and specimen on a microscope slide. Make a thin smear which varies in thickness. Prepare a positive control in the same way.

6 Allow the smear to dry until it appears 'sticky', about 10min.

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