Direct Slide Technique

Gloves are used at all stages

1 With a sterile scalpel cut the tissue into thin slices. Use forceps to hold the tissue. Place the tissue onto a glass slide and squash the pieces with another slide.

2 Hold the slides together with adhesive tape applied at the ends.

3 Examine the preparation under the microscope using the 10* objective and reduced light.

4 If no larvae are seen, a drop of glycerine applied between the slides clears the tissue making any larvae present easier to observe.

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