Cell culture

Most commonly coverslip cultures in 24-well plates are used. After sample inoculation, the cells are incubated for four days. The cultures are then stained with rabbit anti-Toxoplasma antibody and then with fluorescent anti-rabbit IgG. Thereafter, the cultures are read by fluorescence microscopy. Cell culture is more rapid than animal culture, especially if the infected cells are identified by immunofluorescence, but cell culture appears to be less sensitive than mouse inoculation (Derouin et al. 1988; Hitt and Filice 1992). Polymerase chain reaction method has greatly replaced culture assays today due to its better sensitivity and its suitability to study samples with non-viable parasites.

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