H2o2

- SV40 transformed human fibroblasts, quantitative PCR, compare mtDNA to a P-globin fragment

- Lesions, strandbreaks, base modification

- No after 200 ^M for 60 min and 3 h recovery, yes after 15 min exposure

(187)

Glucoseoxidase

- Human fibroblasts, quantitative PCR, compare mtDNA to 3 nuclear genes

- See above

- Yes, 50% by 1 h, 100% by 6h

(135)

Methylnitrosourea (MNU)

- Human fibroblasts (XPD and WI 38 cells), quantitative Southern blot

- N-methylpurine

- Yes, different rates in the two cell types: 77% within 24 h in XPD and 44% in WI 38 cells

(81)

Methylenblue (photoactivated)

- Human fibroblasts in culture, FPG

- 8-oxo-dG

- Yes, 47% by 9 h, no differences between mtDNA strands

n-ethyl-N-nitrosourea

- Rat mitochondria from liver, kidney and brain., immuno-analytical measuring

- O6-ethyl-2'-deoxyguanosine

- Yes, active removal from rat liver, moderate from kidney, nearly no removal from brain mitochondria

(137)

Nitrogen mustard

- CHO cells

- Bulky DNA adducts

- No

(80)

N-nitrosidimethylamine (NDMA)

- Rat

- O6-butyl-2'-deoxyguanosine (O6-DudG) and O6-methyl-2'-deoxyguanosine (O6-MedG)

- Yes, same rate than in nuclear DNA for O6-MedG, much slower for O6-BudG

(108)

4-nitroquinole 1-oxide

- CHO cells, Southern blot

- 4NQO adducts

- Yes

(155)

Psoralen (+ UV light)

- CHO cells, comparison of damage to mtDNA and the nuclear DHFR gene by crosslinking-specific southern blotting

- Interstrand crosslinks

- No

(36)

Streptozotocin

- Rat insulinoma cells

- Alkali-labile sites

- Yes, 70% by 24 h

(122)

UV-irradiation

- Hela cells

- CPDs

- No

(28)

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