Apoptosis can occur in most cells given an appropriate cell death stimulus. Treatment and agents such as serum starvation, staurosporine, and etoposide have been common means of inducing apoptosis in a variety of different cell types. For our purpose, we use Jurkat T cells treated with either 25 ng/ml Fas ligand to induce the extrinsic or death receptor pathway or 30 mJ/cm2 UV to induce the intrinsic or mitochondrial pathway. Jurkat T cells treated with these stimuli are incubated at 37°, 7% CO2 atmosphere for 3 h prior to initiating the specific protocols outlined. In general, any cell type and apoptotic stimulus can be used with these protocols.
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