Preparation of Cens Undergoing Apoptosis

Apoptosis can occur in most cells given an appropriate cell death stimulus. Treatment and agents such as serum starvation, staurosporine, and etoposide have been common means of inducing apoptosis in a variety of different cell types. For our purpose, we use Jurkat T cells treated with either 25 ng/ml Fas ligand to induce the extrinsic or death receptor pathway or 30 mJ/cm2 UV to induce the intrinsic or mitochondrial pathway. Jurkat T cells treated with these stimuli are incubated at 37°, 7% CO2 atmosphere for 3 h prior to initiating the specific protocols outlined. In general, any cell type and apoptotic stimulus can be used with these protocols.

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