Plant Gene Networks in Osmotic Stress Response From Genes to

Regulatory Networks 109

Lam-Son Phan Tran, Kazuo Nakashima, Kazuo Shinozaki, and Kazuko Yamaguchi-Shinozaki

1. Introduction 110

2. Identification of Stress-Responsive Genes 113

3. Identification of cis-Acting Regulatory Elements:

Use of GUS, LUC, and GFP Reporter Genes 115

4. Identification of Trans-Acting Regulatory Elements 118

5. Characterization ofTFs 121

6. Functional Analysis of TFs In Planta 122 Acknowledgments 124 References 125

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