M

MAPK, see Mitogen-activated protein kinase Mechanosensitive channels, bacteria, see also Osmosensing channels, bacteria gain-of-function mutant evaluation, 57-59 hypoosmotic challenge assay cell culture, 54-55 principles, 49-54 variations, 56 viability assay, 55 loss-of-function mutant evaluation, 5-58 osmosensing, 82 overview, 47-49 Membrane potential, Cell Lab Quant SC determination in apoptotic volume decrease, 171-172, 177, 179-180

Membrane ruffling, hyperosmolarity induction,

349-350 MEN, see Mitotic exit network Mitogen-activated protein kinase anhydrosignaling, 271 bile secretion regulation, 317-318 classification, 298

hepatic autophagic proteolysis regulation, 137 high osmolarity glycol signal transduction,

34-35, 39, 64 osmostress response, see also Stress-activated protein kinase fluorescence resonance energy transfer of scaffold members cell culture, 305-306 data interpretation, 309-310 efficiency calculations, 308-309 fluorescent chimeric protein construction, 304-305 image acquisition, 307 microscope configuration, 306 principles, 303-304 spectral cross talk coefficient determination, 308 MEKK3 assay, 302-303 overview, 298-300 p38 mitogen-activated protein kinase assay, 303 RNA interference analysis electroporation, 301 overview, 300-302 transfection, 301-302 plant salinity stress response, 430 Mitotic exit network, stress-activated protein kinase regulation, 82 Molecular dynamics simulation, macromolecules in protective and denaturing osmolytes chymotrypsin inhibitor 2 starting configurations, 376 trimethylamine N-oxide solution, 389-392 urea solution, 386, 389-392 water solution, 386 hydrogen bonding structure of first water coordination shell, 379, 383 overview, 374-375, 377 pure water model, 379 trajectories, 377-378 trimethylamine N-oxide solution modeling, 382-386 starting configurations, 376 urea potential function parameters, 376 solution modeling, 379-381 starting configurations, 376 Msn2, high osmolarity glycol signal transduction, 36-37 Msn4, high osmolarity glycol signal transduction, 36-37

M value

Kirkwood-Buff analysis equation derivation, 474—476 interpretation, 476—479 transfer-free energy model prediction, 414-415

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