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the RBC in the native isotonic region. RBC hydration depends on the henc = 1.7 g/g of hemoglobin plus the water content allowed by response to the osmotic pressure of the nonpenetrating osmolytes outside the cell hos «0.4 g/g or a total RBC water content hRBC ~2.1 g/g or 68% water. Thus 1.7/2.1 = 81% of the water in the RBC is water encapsulated by the hemoglobin, compelling evidence that protein hydration must be considered when evaluating tissue osmoregulation.

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