General considerations

A combination of volume measurements and patch clamp can be used on larger cells (e.g., epithelial or endothelial cells) adhering to a coated coverslip. The general problem of using whole cell patch clamp in conjunction with hypotonic cell swelling has already been discussed in detail. It should be carefully taken into account that the cell volume of the patched cells increased constantly and never reached a steady state and evaluated how much this unavoidable pitfall influences the conclusions drawn by the experimentalist.

Was this article helpful?

0 0

Post a comment