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Gel filtration, TonE-binding protein, 265 GFP, see Green fluorescent protein Glucose transport hyperosmolarity effects on 3T3-L1 adipocytes differentiation, 347 glucose uptake induction, 347-348 insulin-stimulated uptake inhibition, 349 phosphatidylinositol 3-kinase analysis activity assay, 353 immunoprecipitaton, 352 substrate preparation, 352 signaling pathways cell lysate preparation, 350-351 immunoprecipitation ofdocking proteins, 351

Glucose transport (cont.)

overview, 345-348, 350 Western blot, 351 insulin-induced GLUT4 translocation,

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