This chapter summarizes the current approaches permitting the study of hyperosmolarity on membrane ruffling, Glut 4 translocation, glucose transport, and signaling pathways in 3T3-L1 adipocytes. It should be noted that hyperosmotic stress (usually induced by high extracellular concentrations of sorbitol) is not a physiological stimulus, but is used as a tool that could lead to the discovery of novel molecular mechanisms of glucose transport and also for a better understanding ofthe molecular mechanisms ofcellular insulin resistance.

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