C

1 nA

5 min

Suramin

Figure 19-1 Volume-sensitive chloride currents from Intestine 407 cells. (A) Whole cell Cl— currents in control (isotonic) and hypoosmotically stimulated cells in response to step pulses (2-s duration) from —100 to 100 mV (25-mV increments). (B) Current-to-voltage relationship of hypotonicity-provoked Cl— currents immediately after the onset of the pulse (circles) and after reaching their plateau values (triangles). (C) Time course of hypotonicity-provoked anion currents at —100 and +100 mV, as well as the voltage-dependent inhibition of the currents by suramin (100 mM).

1 nA

5 min

Suramin

Figure 19-1 Volume-sensitive chloride currents from Intestine 407 cells. (A) Whole cell Cl— currents in control (isotonic) and hypoosmotically stimulated cells in response to step pulses (2-s duration) from —100 to 100 mV (25-mV increments). (B) Current-to-voltage relationship of hypotonicity-provoked Cl— currents immediately after the onset of the pulse (circles) and after reaching their plateau values (triangles). (C) Time course of hypotonicity-provoked anion currents at —100 and +100 mV, as well as the voltage-dependent inhibition of the currents by suramin (100 mM).

To monitor the development of the current, alternating step pulses (100-ms duration) from 0 to 100 mV are applied every 30 s. Voltage dependence of whole cell current is monitored by applying step pulses (2-s duration, 7-s interval) from —100 to +100 mV with 25-mV increments (Fig. 19.1). For command pulse control, data acquisition, and analysis, pCLAMP 9 software (Axon Instruments, Union City, CA) was used. All data are sampled at 5 kHz after being low pass filtered at 500 Hz.

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