N

Neonatal care cloned cattle, 95 Nuclear potential historical, 10-11 amphibians, embryonic cells, 15-16 somatic cells, 16-18 Nuclear reprogramming aberrant, 309

activation. See Oocytes, activation amphibian cloning, 20-22 applications, 233-234 assessment, 266 cell hybridization, 230-232 gene expression, 287-289 in vitro, 259 overview, 229-236

nuclear transfer, 22-23, 23, 23-24, 11-15, 61-67, 115-118, 129, 222-224, 140-147, 159-161, 175-177, remodelling assay, 252-253 spontaneous cell fusion, 232-233 induction 230-231 Nuclear transplantation abnormalities, 309, 312-315 advances, historical, 5-6 applications, overview, 319-331 platform technology, 320 domestic species, 321 wildlife conservation, 323 production of ES cells, 324 models of human disease, 327 ascidians, 23 cattle, 129

chromosome studies, 19 Desert Bighorn Sheep, 175-177 efficiency, 321 embryonic donor cells, 309 fish, 23-24 frogs, 11-15 goats, 222-224 interspecies, 327 insects, 22-23

large offspring syndrome (LOS), 287

mouse, 67-71, 115-118

monkeys, 159-161

pigs, 140-147

somatic donor cells, 309

telomeres, 101, 315

to mouse embryonic stem cells, 278

to mouse embryonic stem cell cytoplasts, 278 xenogenic, 327 Nucleo-cytoplasmic hybrids, 23

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