Pzw

Karyotypic analysis, 203 L

Large offspring syndrome (LOS), 287 M

M-phase promoting factor (MPF), 48, 166

inhibiting activity, 48-53 mRNA determination from single embryos, 293-294 Mammal cloning. See also specific mammals abnormalities, 309, 312-315 advances historical, 5-6 applications, overview, 319-331 platform technology, 320 domestic species, 321 wildlife conservation, 323 production of ES cells, 324 models of human disease, 327 ascidians, 23 cattle, 129

Desert Bighorn Sheep, 175-177

efficiency, 321

embryonic donor cells, 309

fish, 23-24

frog, 11-15

goats, 222-224

insects, 22-23

mouse, 67-71, 115-118 monkey, 159-161 pig, 140-147 somatic donor cells, 309 xenogenic, 327 Markers drug selection, 202, 218-220 frog cloning, 14 monoclonal mice, 18-19 T-cell specific, 267 Metaphase III, 166 Methylation patterns bisulphite sequencing, 294-298 determination in single embryos, 294 in cloned embryos, 289-290 in in vitro produced embryos, 289-290 overview, 285-303 Micromanipulation methods, historical, 10-14 Monkey. See Rhesus macaque; primates Mouse cloning procedure, 114-118 differential staining of embryos, 73-74 in vitro culture, 72, 113-114 nuclear transplantation, 76-71, 115-118 oocyte activation, 46, 47, 71 zygote culture test, 66

Was this article helpful?

0 0

Post a comment