282 ± 10
Filter the medium using a 0.2-|j. filter unit and store for up to one month at +4°C. Add BSA (Sigma) at 3 mg/mL before use and refilter. 5. Hamster embryo culture medium (HECM)-9 (7): Prepare HECM-9 base medium by adding the indicated amounts of each reagent (Sigma) to 1 L of Milli-Q water. Polyvinyl alcohol (PVA) 0.1 g
CaCl2-2H2O MgCl2-6H[2O NaHCO3
Lactic acid, Na salt, 60% syrup Gentamicin sulfate pH
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